Sandwich ELISA

To remember this type of ELISA, imagine a sandwich! The antigen is the filling and the antibodies are the yummy bread. Hungry, aren't you?

Sandwich ELISA

Indirect ELISA

Indirect ELISA is used for detection of a particular antibody in a given sample. Most of the HIV diagnosis kits use indirect ELISA principle.

Indirect ELISA

Direct ELISA

Direct ELISA is performed by attaching the sample antigens on a surface (walls of wells) then a specific antibody is applied so that it can bind to its corresponding antigen.

Direct ELISA

ELISA: Enzyme Linked Immuno Sorbent Assay

ELISA: Enzyme Linked Immuno Sorbent Assay

From pregnancy detection kits to HIV diagnosis it is ELISA which gives us appropriate results.  

Though ELISA has got a complex name, the way in which it diagnosis is really simple!

ELISA is performed in special micro titre plates which has wells in which we add our sample and then perform ELISA.

There are 4 types of ELISA:

- Direct

- Indirect

- Sandwich
- Competitive

We’ll explain them one by one over the next few blogs. So stay tuned! :)

Written by Komal M. Kadam

Policemen of our body

Just like policemen save us from criminals, immune cells save our body!

T cell